Coenzyme A Trilithium Salt

CAS No:	18439-24-2
EINECS No:	205-745-7
Synonyms:	Coenzyme A trilithium salt from yeast, CoA-Li, Co A-Li, Co A-SH, CoA trilithium salt, Trilithium Coenzyme A, Trilithium salt of CoA

Product Summary

Coenzyme A trilithium salt is a highly soluble, bioactive lithium salt form of Coenzyme A (CoA), a vital cofactor in numerous metabolic and enzymatic processes. This purified form is especially favored in biochemical research, enzymology, and pharmaceutical applications due to its enhanced stability and compatibility with aqueous systems.

Formula: C₂₁H₃₃N₇O₁₆P₃S · 3Li
Formula Molecular Weight: 785.33 (as anhydrous trilithium salt)
Source: Yeast
Lithium content: 1.5% - 4.5%
Water: Not more than 8%
Purity: Not less than 90%

Function

Coenzyme A trilithium salt acts as a central acyl group carrier in metabolism. It facilitates the formation of thioester bonds with organic acids, converting them into activated acyl-CoA derivatives such as acetyl-CoA, succinyl-CoA, and malonyl-CoA. These intermediates are essential in:

The citric acid (TCA) cycle
Fatty acid synthesis and oxidation
Amino acid metabolism
Ketone body production
Cholesterol biosynthesis

Mechanism of Action

At the molecular level, CoA features a reactive thiol group (-SH) at its terminus that forms high-energy thioester bonds with acyl groups. This activated form acts as a substrate or cofactor for many enzymes, driving acyl-transfer reactions crucial to cellular metabolism.

The trilithium salt form ensures:

Enhanced solubility in aqueous buffers
Stability under standard storage and assay conditions
Compatibility with enzyme assays and cell-based studies

Applications in Scientific Research

Used to study CoA-dependent enzymes like acetyl-CoA synthetase, pyruvate dehydrogenase, and fatty acid synthase.
Essential in in vitro reconstitution of metabolic pathways.
Investigated in studies of protein CoAlation as a novel oxidative stress response.
Screening CoA analogs as enzyme inhibitors or metabolic modulators.
Applied in synthetic biology and enzyme-driven transformations.
Research into energy metabolism and mitochondrial function.
CoA derivatives used in assays to monitor enzyme activity and metabolic flux.

Packaging & Storage

Available as white or slightly yellow lyophilized powder
Store at -20°C in tightly sealed containers, protected from moisture and light