Beta-Glucanase refers to a group of enzymes derived from Saccharomyces cerevisiae that catalyze the hydrolysis of β-glucans, which are polysaccharides composed of β-D-glucose units linked primarily by β-1,3 and/or β-1,4 glycosidic bonds. These enzymes play a vital role in breaking down complex glucans into simpler sugars.
Beta-Glucanase
| CAS No: | 9075-79-2 |
| EINECS No: | 232-627-2 |
| EC No: | 3.2.1.6 |
| Synonyms: | endo-1,3(4)-β-glucanase, β-1,3-1,4-glucanase, lichenase, β-glucan hydrolase, β-D-glucanase, 1,3-1,4-β-D-glucan 4-glucanohydrolase, mixed-linkage β-glucanase |
Product Summary
Function
Beta-glucanase hydrolyzes β-glucans—complex polysaccharides found in the cell walls of cereals (like barley and oats), fungi, and yeasts. It converts these glucans into simpler, more digestible sugars such as oligosaccharides or glucose.
- Optimal pH: 5.0 – 6.0
- Optimal Temperature: 45°C – 55°C
Mechanism of Action
Beta-glucanase breaks internal β-1,3 and β-1,4 glycosidic bonds in glucan polysaccharides. It may also catalyze transglycosylation, forming new β-glycosidic bonds between donor and acceptor saccharides.
Applications in Scientific Research
- Cell wall degradation studies in fungi and yeasts
- Plant-microbe interaction analysis
- Immunological studies involving β-glucans
Packaging & Storage
- Available as lyophilized powder
- Store at 2–8°C (powder); –20°C recommended for long-term preservation
References
- Keitel T. et al. 1993: Molecular and active-site structure of a Bacillus 1,3-1,4-β-glucanase, PNAS 90(11): 5287–91.
- Linton SM. 2019: Review: The structure and function of cellulase and hemicellulase enzymes in invertebrates, Comp Biochem Physiol B 240:110354.
- Borriss R. 1990: Structure of the β-1,3-1,4-glucanase gene of Bacillus macerans: homologies to other β-glucanases, Mol Gen Genet. 222(2-3):278–83.
- Heng NC. 1997: Cloning and expression of an endo-1,3-1,4-β-glucanase gene from Bacillus macerans in Lactobacillus reuteri, Appl Environ Microbiol. 63(8):3336–40.
- Flint HJ. et al. 1989: Molecular cloning of genes from Ruminococcus flavefaciens encoding xylanase and β(1-3,1-4)glucanase activities, Appl Environ Microbiol. 55(5):1230–3.
- Celestino KRS. 2006: Characterization of a β-glucanase produced by Rhizopus microsporus var. microsporus, BMC Biochem. 7(23).
- Wang G. et al. 1999: Development of a 96-well enzyme-linked solid-phase assay for β-glucanase and xylanase, J Agri Food Chem. 47(3).
- Sarkar N. et al. 2023: Beta-glucans in biotechnology: a holistic review with a special focus on yeast, Bioengineering. 12(4):365.
- Wang K. et al. 2023: Expression, purification and characterization of endo-β-1,3-glucanase from Penicillium expansum, J Fungi. 9(10):961.
- Sun JB. et al. 2020: Directed mutation of β-glucanases from probiotics to enhance enzymatic activity, thermal and pH stability, Arch Microbiol. 202:1949–56.
- Chobotova K. et al. 2022: Emerging roles of β-glucanases in plant development and adaptive responses, Plants. 11(9):1119.
- Jin X. et al. 2023: Microbial β-glucanases: production, properties, and engineering, World J Microbiol Biotechnol. 39(4):106.