- Hyaluronidase cleaves the β-1,4-glycosidic bonds between N-acetyl-D-glucosamine and D-glucuronic acid units in hyaluronic acid.
- Optimal conditions (bovine, ovine, or recombinant source):
- pH: ~4.5–5.5
- Temperature: ~37°C
Hyaluronidase
| CAS No: | 9001-54-1 |
| EINECS No: | 232-799-9 |
| EC No: | 3.2.1.35 |
| Synonyms: | hyaluronate lyase, hyase, hyase-1, hyalase, mucolase |
Product Summary
Hyaluronidase is a hyaluronic acid-degrading enzyme that catalyzes the hydrolysis of hyaluronic acid (HA) and related glycosaminoglycans. This enzyme plays a pivotal role in modulating the extracellular matrix (ECM), enhancing tissue permeability, and facilitating molecular diffusion.
Biochemical Function & Mechanism of Action
Applications in Scientific Research
- Used to model ECM remodeling and investigate cell migration, cancer metastasis, and wound healing.
- Enhances the permeability of subcutaneous or intradermal tissues, facilitating delivery of chemotherapeutics, gene therapies, and nanoparticles.
- Employed to dissociate tissues for primary cell culture preparation (e.g., adipose-derived stem cells).
- Used to modulate intraocular pressure and as a model enzyme in skin aging and ECM degradation studies.
Packaging & Storage
- White or almost white lyophilized powder
- Store at 2–8°C, or at –20°C for long-term storage
References
- Meyer K, Rapport MM. 1952: Hyaluronidases, Ed: Nord FF.
- Kreil G. 1995: Hyaluronidases--a group of neglected enzymes, Protein Sci. 4(9):1666–9.
- Menzel EJ, Farr C. 1998: Hyaluronidase and its substrate hyaluronan: biochemistry, biological activities and therapeutic uses, Cancer Lett. 131(1):3–11.
- Stern R, Jedrzejas MJ. 2006: The hyaluronidases: their genomics, structures, and mechanisms of action, Chem Rev. 106(3):818–39.
- Li S, et al. 2000: Structural basis of hyaluronan degradation by Streptococcus pneumoniae hyaluronate lyase, EMBO J. 19(6):1228–40.
- Stern R. 2008: Hyaluronidases in cancer biology, Semin Cancer Biol. 18(4):275–80.
- Jedrzejas MJ, Stern R. 2005: Structures of vertebrate hyaluronidases and their unique enzymatic mechanism of hydrolysis, Proteins. 61(2):227–38.
- Chao KL, et al. 2007: Structure of human hyaluronidase-1, a hyaluronan hydrolyzing enzyme involved in tumor growth and angiogenesis, Biochem. 46(23):6911–20.
- Wang Z, et al. 2014: Two novel functions of hyaluronidase from Streptococcus agalactiae are enhanced intracellular survival and inhibition of proinflammatory cytokine expression, Infect Immun. 82(6):2615–25.
- Girish KS, et al. 2017: Hyaluronidase inhibitors: a biological and therapeutic perspective, Curr Med Chem. 16(18):2261–88.
- Jiang JY, et al. 2024: A comprehensive review on microbial hyaluronidases: from virulence factors to biotechnology tools, Bioresour Bioprocess. 11(1):114.
- DeRossi R, et al. 2011: Hyaluronidase shortens levobupivacaine lumbosacral epidural anaesthesia in dogs, J Small Anim Pract. 52(4):195–9.