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Gastric Mucin
HCG
FSH
UTI
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Aprotinin
Asparaginase
Beta Glucanase
Bromelain
Chymotrypsin
Kallikrein
Lysozyme
Pancreatin
Papain
Pepsin
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Trypsin
Urokinase
Coenzyme A
Coenzyme A Trilithium
Coenzyme Q10
Beta Glucans
Chitosan
Chondroitin Sulfate
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Dextran Sulfate Sodium
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Rosemary Extract
Sodium Cholate
Bilirubin
Cytochrome C
Gastric Mucin
HCG
FSH
UTI
PMSG
Aprotinin
Asparaginase
Beta Glucanase
Bromelain
Chymotrypsin
Kallikrein
Lysozyme
Pancreatin
Papain
Pepsin
Streptokinase
Trypsin
Urokinase
Coenzyme A
Coenzyme A Trilithium
Coenzyme Q10
Beta Glucans
Chitosan
Chondroitin Sulfate
Dextran
Dextran Sulfate Sodium
Glucosamine HCl
Heparin Sodium
Heparinoid
Hyaluronic Acid
Rosemary Extract
Sodium Cholate
Jiagen Biotechnologies
Enzymes

Lysozyme

CAS No:9001-63-2
EINECS No:232-620-4
EC No:3.2.1.17
Synonyms:Muramidase, N-acetylmuramide glycanhydrolase, egg-white lysozyme, peptidoglycan N-acetylmuramoylhydrolase

Product Summary

Lysozyme is a naturally occurring antibacterial enzyme that plays a vital role in the innate immune system. It catalyzes the hydrolysis of β-1,4-glycosidic bonds in the peptidoglycan layer of bacterial cell walls, leading to cell lysis. It is especially effective against Gram-positive bacteria.

Biochemical Function & Mechanism of Action

Lysozyme targets the polysaccharide component of bacterial cell walls—specifically the bonds between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) in peptidoglycan. The enzymatic cleavage compromises the structural integrity of the cell wall, causing osmotic imbalance and cell death.

  • Optimal pH: 6.0 - 7.0
  • Active Temperature: 20°C – 37°C

Applications in Scientific Research

  • Used in molecular biology for cell lysis to extract DNA, RNA, and proteins.
  • Helps prepare protoplasts from bacterial cells.
  • Used as a marker enzyme in enzymatic activity assays.
  • Used for cell wall degradation in recombinant protein production.

Packaging & Storage

  • Available as lyophilized powder
  • Store at 2–8°C

References

  1. Blake CC, et al. 1965: Structure of hen egg-white lysozyme. A three-dimensional Fourier synthesis at 2 Angstrom resolution , Nature 206(4986):757–61.
  2. Philips DC. 1967: The hen egg white lysozyme molecule , Proc Natl Acad Sci USA. 57(3):483–95.
  3. Koshland DE. 1953: Stereochemistry and mechanism of enzymatic reactions , Biol Rev. 28(4):416–36.
  4. Vocadlo DJ. 2001: Catalysis by hen egg white lysozyme proceeds via a covalent intermediate , Nature 412:835–8.
  5. Chipman DM, et al. 1968: Lysozyme-catalyzed hydrolysis and transglycosylation reactions of bacterial cell wall oligosaccharides , J Biol Chem. 243(3):487–96.
  6. Holler E. 1975: Productive and unproductive lysozyme-chitosaccharide complexes. Kinetic investigations , Biochem. 14(11):2377–85.
  7. Derde M, et al. 2013: Hen egg white lysozyme permeabilizes Escherichia coli outer and inner membranes , J Agric Food Chem. 61(41):9922–9.
  8. Wei Z, et al. 2021: Enhanced antibacterial activity of hen egg-white lysozyme against Staphylococcus aureus and Escherichia coli due to protein fibrillation , Biomacromolecules. 22(2):890–7.
  9. Strazdaite S, et al. 2020: Structure determination of hen egg-white lysozyme aggregates adsorbed to lipid/water and air/water interfaces , Langmuir 36(17):4766–75.
  10. Zhang Q, et al. 2024: Characteristics of hen egg white lysozyme, strategies to break through antibacterial limitation, and its application in food preservation: A review , Food Res Int. 181:114114.
  11. Bergamo A, Sava G. 2023: Pharmacological modulation of host immunity with hen egg white lysozyme (HEWL) – A review , Molecules. 28(13):5027.
  12. Lerbret A, et al. 2007: How do trehalose, maltose and sucrose influence some structural and dynamical properties of lysozyme? An insight from Molecular Dynamics simulations , J Phys Chem B. 111(31):9410–20.

Jiagen Biotechnologies supplies quality Lysozyme of various specifications.
Contact us at sales@jiagen.ca to place an order.

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